TEAD4 Recombinant antibody, PBS Only (Detector)

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  WB analysis of MDA-MB-231 using 84932-4-RR (same clone as 84932-4-PBS)

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 84932-4-RR (TEAD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84932-4-PBS in a different storage buffer formulation.

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  WB analysis of BGC-823 using 84932-4-RR (same clone as 84932-4-PBS)

  BGC-823 cells were subjected to SDS PAGE followed by western blot with 84932-4-RR (TEAD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84932-4-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01682-3

  Sandwich ELISA standard curve of MP01682-3, Human TEAD4 Recombinant Matched Antibody Pair - PBS only. 84932-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3059. 84932-4-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.

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  Sample test of MP01682-3

  The mean TEAD4 concentration was determined to be 8.65 ng/mL in HepG2 cell extract based on a 1.00 mg/mL extract load and 6.31 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load.

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  IF Staining of HeLa using 84932-4-RR (same clone as 84932-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TEAD4 antibody (84932-4-RR, Clone: 242496B2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84932-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84932-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84932-4-RR against Human TEAD4 were performed. The affinity constant is 0.205 nM.

TEAD4 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84932-4-PBS

Clone No.242496B2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

TEA domain family member 4, TCF13L1, RTEF1, hRTEF 1B, EFTR 2

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Product Information

84932-4-PBS targets TEAD4 as part of a matched antibody pair:

MP01682-3: 84932-5-PBS capture and 84932-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TEAD4 fusion protein Ag3059
• Full Name: TEA domain family member 4
• Calculated Molecular Weight: 434 aa, 48 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC015497
• Gene Symbol: TEAD4
• Gene ID (NCBI): 7004
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15561
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

TEAD4 belongs to a protein family that shares the TEA/ATTS domain. The transcriptional activity of TEAD4 required several regions of the proteins, a TBP-associated factor, and a limiting transcriptional intermediary factor. TEAD4 also performs an essential role in the Hippo signaling pathway. TEAD4 regulates gene expression of YAP1 and WWTR1/TAZ, thus mediating cell proliferation, migration, and epithelial-mesenchymal transition induction. The calculated molecular weight of TEAD4 is 48 kDa, but the molecular weight of TEAD4 is detected at about 50-55 kDa.