TEAD3 Polyclonal antibody

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  WB analysis using 13120-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13120-1-AP (TEAD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse small intestine using 13120-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 13120-1-AP (TEAD3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 13120-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 13120-1-AP (TEAD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

TEAD3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 13120-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

TEAD-3, TEAD 3, TEA domain family member 3, ETFR 1, DTEF-1

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Tested Applications

• Positive WB detected in: HCT 116 cells, HepG2 cells, MCF-7 cells
• Positive IHC detected in: mouse small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below

Product Information

13120-1-AP targets TEAD3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TEAD3 fusion protein Ag3875
• Full Name: TEA domain family member 3
• Calculated Molecular Weight: 435 aa, 49 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC027877
• Gene Symbol: TEAD3
• Gene ID (NCBI): 7005
• RRID: AB_2203068
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99594
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TEAD3, others named ranscriptional enhancer factor 5(TEF5), a member of family of transcription factors that plays a key role in the Hippo signaling pathway, suggesting involve in organ and tumor suppression by restricting proliferation and promoting. TEAD3 contains the TEA/ATTS DNA-binding domain. Human TEAD3 has a strong mRNA expression in placenta and choriocarcinoma cells(JEG-3), but low in Hela, HepG2 and MCF-7. This is a rabbit polyclonal anti-TEAD3 antibody raised against part of C-terminal chain of human TEAD3.

Publications

Species	Application	Title
mouse	WB	J Cell Biol; Thyroid hormone regulates muscle fiber type conversion via miR-133a1.; Authors - Duo Zhang
mouse	WB	Res Sq; Long noncoding RNA Malat1 inhibits Tead3-Nfatc1-mediated osteoclastogenesis to suppress osteoporosis and bone metastasis; Authors - Yang Zhao
human	WB	Sci Adv; TEAD4 is a master regulator of high-risk nasopharyngeal carcinoma; Authors - Ya-Qin Wang
mouse	IHC	Adv Sci (Weinh); SUMOylation of TEAD1 Modulates the Mechanism of Pathological Cardiac Hypertrophy; Authors - Xin Shi
mouse	WB	Nat Commun; Long noncoding RNA Malat1 protects against osteoporosis and bone metastasis; Authors - Yang Zhao
human,mouse	WB	J Med Chem; Selective Degradation of TEADs by a PROTAC Molecule Exhibited Robust Anticancer Efficacy In Vitro and In Vivo; Authors - Yuhang Lu