TEAD2 Polyclonal antibody

• Featured Product
• KD/KO Validated

TEAD2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ChIP, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 21159-1-AP

Synonyms

ETF, TEA domain family member 2, TEAD 2, TEAD2, TEF 4, TEF4

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Validation Data Gallery

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  WB analysis of mouse colon using 21159-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 21159-1-AP (TEAD2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 21159-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21159-1-AP (TEAD2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse colon tissue, mouse lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• ChIP: See 1 publications below

Product Information

The immunogen of 21159-1-AP is TEAD2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TEAD2 fusion protein Ag14074
• Full Name: TEA domain family member 2
• Calculated Molecular Weight: 447 aa, 49 kDa
• Observed Molecular Weight: 55-65 kDa
• GenBank Accession Number: BC051301
• Gene Symbol: TEAD2
• Gene ID (NCBI): 8463
• RRID: AB_2861186
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TEAD2 belongs to a protein family that share the TEA/ATTS domain. It acts as a transcriptional factor by cooperatively binding to the GT-IIC and Sph(I 1 II) enhansons in the simian virus 40 (SV40) enhancer. Also it can modulate SV40 late transcription together with large T antigen. The transcriptional activity of TEAD2 required several regions of the proteins , a TBP-associated factors and a limiting transcriptional intermediary factors. TEAD2 also preforms an essential role in the Hippo signaling pathway. TEAD2 regulates gene expression of YAP1 and WWTR1/TAZ, thus mediating cell proliferation, migration and epithelial mesenchymal transition induction. TEAD2 is strongly expressed in human ovarian carcinoma cell line.

Publications

Species	Application	Title
human,rat	WB	ACS Nano; Hepatic Stellate Cell- and Liver Microbiome-Specific Delivery System for Dihydrotanshinone I to Ameliorate Liver Fibrosis; Authors - Xia Niu
mouse	WB	J Cell Biol; Thyroid hormone regulates muscle fiber type conversion via miR-133a1.; Authors - Duo Zhang
human	WB	Aging (Albany NY); Integrative bioinformatics and experimental analysis revealed TEAD as novel prognostic target for hepatocellular carcinoma and its roles in ferroptosis regulation.; Authors - Xinxin Ren ; KD Validated
human	WB	Oncol Rep; 17‑AAG synergizes with Belinostat to exhibit a negative effect on the proliferation and invasion of MDA‑MB‑231 breast cancer cells.; Authors - Yu Zuo
human	WB	Int J Gen Med; Significance of TEAD Family in Diagnosis, Prognosis and Immune Response for Ovarian Serous Carcinoma.; Authors - Xinxin Ren ; KD Validated
mouse	WB	Cell Rep; YTHDF2/3 Are Required for Somatic Reprogramming through Different RNA Deadenylation Pathways.; Authors - Jiadong Liu