TEAD1 Polyclonal antibody

• Featured Product
• KD/KO Validated

TEAD1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 13283-1-AP

Synonyms

AA, NTEF 1, Protein GT IIC, REF1, TCF 13, TCF13, TEA domain family member 1, TEAD 1, TEAD1, TEF 1, TEF1, Transcription factor 13

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Validation Data Gallery

• 

  WB analysis of BGC-823 using 13283-1-AP

  BGC-823 cells were subjected to SDS PAGE followed by western blot with 13283-1-AP (TEAD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 13283-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 13283-1-AP (TEAD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 13283-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 13283-1-AP (TEAD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13283-1-AP

  C2C12 cells were subjected to SDS PAGE followed by western blot with 13283-1-AP (TEAD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: BGC-823 cells, A375 cells, C2C12 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IF: See 1 publications below

Product Information

13283-1-AP targets TEAD1 in WB, IF, ELISA applications and shows reactivity with mouse, human samples.

• Tested Reactivity: mouse, human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TEAD1 fusion protein Ag4082
• Full Name: TEA domain family member 1 (SV40 transcriptional enhancer factor)
• Calculated Molecular Weight: 426 aa, 47, 9 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC026959
• Gene Symbol: TEAD1
• Gene ID (NCBI): 7003
• RRID: AB_2287290
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TEAD1 belongs to a protein family that share the TEA/ATTS domain. It acts as a transcriptional factor by binding to the GT-IIC and Sph(I 1 II) enhansons in the simian virus 40 (SV40) enhancer. Also it can modulate SV40 late transcription together with large T antigen. The transcriptional activity of TEAD1 required several regions of the proteins , a TBP-associated factors and a limiting transcriptional intermediary factors. TEAD1 also preforms an essential role in the Hippo signaling pathway. TEAD1 regulates gene expression of YAP1 and WWTR1/TAZ, thus mediating cell proliferation, migration and epithelial mesenchymal transition induction. TEAD is preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart.

Publications

Species	Application	Title
human	WB	Cancer Lett; EGFR/c-myc axis regulates TGFβ/Hippo/Notch pathway via epigenetic silencing miR-524 in gliomas.; Authors - Kai Zhao
human	WB	Cancers (Basel); WISP2/CCN5 Suppresses Vasculogenic Mimicry through Inhibition of YAP/TAZ Signaling in Breast Cancer Cells.; Authors - Nathalie Ferrand
human	WB	Int J Mol Sci; Role of Clostridium perfringens Enterotoxin on YAP Activation in Colonic Sessile Serrated Adenoma/ Polyps with Dysplasia.; Authors - Rina Fujiwara-Tani
human	WB,IF	Dig Dis Sci; TEA Domain Transcription Factor 1 Inhibits Ferroptosis and Sorafenib Sensitivity of Hepatocellular Carcinoma Cells; Authors - Hongmin Li
human	WB	Biomed Pharmacother; YAP1-TEAD1-Glut1 axis dictates the oncogenic phenotypes of breast cancer cells by modulating glycolysis.; Authors - Chunli Lin
	WB	Oncol Rep; YAP is overexpressed in clear cell renal cell carcinoma and its knockdown reduces cell proliferation and induces cell cycle arrest and apoptosis.; Authors - Jian-Jia Cao