Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.
Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.
WB analysis using 66845-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 66845-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of NCCIT using 66845-1-Ig
NCCIT cell were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
NCCIT cell were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of LNCaP using 66845-1-Ig
LNCaP cells were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
LNCaP cells were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 66845-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66845-1-Ig (TDRKH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66845-1-Ig (TDRKH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 66845-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66845-1-Ig (TDRKH antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66845-1-Ig (TDRKH antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
rat brain tissue, JAR cells, NCCIT cells, LNCaP cells, pig brain tissue, mouse brain tissue, rabbit brain tissue, rat testis tissue, mouse testis tissue, Rat brain
Positive IHC detected in
mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:10000
Immunohistochemistry (IHC)
IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
66845-1-Ig targets TDRKH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Tudor and KH domain-containing protein(TDRKH)Tudor domains are protein modules that mediate protein-protein interactions, potentially by binding to methylated ligands. Both KH and tudor domains are involved in binding RNA or single-strand DNA. Tudor also is a germ cell-specific protein with multiple Tudor domains and is involved in germ plasm formation and germ cell specification. TDRKH is one Tudor protein with a single Tudor domain.
Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.
WB analysis using 66845-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 66845-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of NCCIT using 66845-1-Ig
NCCIT cell were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of LNCaP using 66845-1-Ig
LNCaP cells were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse testis using 66845-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66845-1-Ig (TDRKH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 66845-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66845-1-Ig (TDRKH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HepG2 using 66845-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66845-1-Ig (TDRKH antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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