TDP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

TDP1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 10641-1-AP

Synonyms

Tyrosyl-DNA phosphodiesterase 1, Tyr-DNA phosphodiesterase 1, Tyr DNA phosphodiesterase 1, EC:3.1.4.-

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Validation Data Gallery

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  WB analysis of MCF-7 using 10641-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10641-1-AP (TDP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10641-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10641-1-AP (TDP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10641-1-AP

  IP result of anti-TDP1 (IP:10641-1-AP, 4ug; Detection:10641-1-AP 1:1000) with HeLa cells lysate 1200ug.

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  IHC staining of human ovary cancer using 10641-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10641-1-AP (TDP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 10641-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10641-1-AP (TDP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 10641-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10641-1-AP (TDP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 10641-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10641-1-AP (TDP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MCF7 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human ovary cancer tissue, human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

10641-1-AP targets TDP1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TDP1 fusion protein Ag0964
• Full Name: tyrosyl-DNA phosphodiesterase 1
• Calculated Molecular Weight: 34 kDa, 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC006083
• Gene Symbol: TDP1
• Gene ID (NCBI): 55775
• RRID: AB_2240520
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tyrosyl DNA phosphodiesterase 1 (TDP1) is an enzyme capable of hydrolyzing phosphodiester bonds between tyrosine and the 3-phosphate of DNA, which are typically generated in a transient manner by DNA topoisomerase I (topo I) and it appears to be more mobile and diffusible than topo I, and it has a different distribution in the nucleus (PMID:15494395). TDP1 is involed in protecting cells against oxidative DNA damage, and with the impaired ability of TDP1-deficient cells to remove 3-phosphoglycolate (PMID:21041670). Defects in TDP1 are the cause of spinocerebellar ataxia autosomal recessive with axonal neuropathy (SCAN1)(PMID:17948061).

Publications

Species	Application	Title
human	IF	Biochem Pharmacol; Role of tyrosyl-DNA phosphodiesterase 1 and inter-players in regulation of tumor cell sensitivity to topoisomerase I inhibition.; Authors - Perego Paola P
human	WB	DNA Repair (Amst); TDP1 is required for efficient non-homologous end joining in human cells.; Authors - Jing Li ; KO Validated
human	WB	Int J Mol Sci; Combined PARP and Dual Topoisomerase Inhibition Potentiates Genome Instability and Cell Death in Ovarian Cancer; Authors - Inken Flörkemeier