TCTN3 Polyclonal antibody

TCTN3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16085-1-AP

Synonyms

C10orf61, TCTN3, TECT3, Tectonic 3, tectonic family member 3, UNQ1881/PRO4324

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Validation Data Gallery

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  WB analysis of mouse liver using 16085-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16085-1-AP (TCTN3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Hela cells using 16085-1-AP

  IP result of anti-TCTN3 (16085-1-AP for IP and Detection).

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  IHC staining of human lung using 16085-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16085-1-AP (TCTN3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 16085-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16085-1-AP (TCTN3 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IHC detected in: human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 2 publications below

Product Information

16085-1-AP targets TCTN3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TCTN3 fusion protein Ag9084
• Full Name: tectonic family member 3
• Calculated Molecular Weight: 607 aa, 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC009494
• Gene Symbol: TCTN3
• Gene ID (NCBI): 26123
• RRID: AB_10638442
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TCTN3 (tectonic-3 or TECT3) is a type I membrane protein that belongs to the tectonic family, which consists of TCTN1, TCTN2 and TCTN3. Studies in mice suggest that tectonic may be involved in Hedgehog (Hh) signaling, and essential for ciliogenesis. Tectonic is component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. TCTN3 is necessary for transduction of the sonic hedgehog (SHH) signaling pathway. Orofaciodigital syndromes (OFDSs) consist of a group of heterogeneous disorders characterized by abnormalities in the oral cavity, face, and digits and associated phenotypic abnormalities. Recently, TCTN3 mutations are found as the cause of an extreme form of OFD associated with bone dysplasia, tibial defect, cystic kidneys, and brain anomalies.

Publications

Species	Application	Title
human	WB,IF	Nat Genet; A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.; Authors - Garcia-Gonzalo Francesc R FR
mouse	IF	EMBO J; Cell type-specific regulation of ciliary transition zone assembly in vertebrates.; Authors - Antonia Wiegering