TCTN2 Recombinant antibody, PBS Only

TCTN2 Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E17

Cat no : 82121-1-PBS

Synonyms

C12orf38, FLJ12975, TCTN2, TECT2, Tectonic 2, tectonic family member 2

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Validation Data Gallery

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  WB analysis using 82121-1-RR (same clone as 82121-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82121-1-RR (TCTN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82121-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82121-1-PBS

  Biolayer interferometry (BLl) kinetic assays of 82121-1-RR against Human TCTN2 were performed. The affinity constant is 4.48 nM.

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Product Information

82121-1-PBS targets TCTN2 in WB, Indirect ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TCTN2 fusion protein Ag10725
• Full Name: tectonic family member 2
• Calculated Molecular Weight: 697 aa, 77 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC009112
• Gene Symbol: TCTN2
• Gene ID (NCBI): 79867
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TCTN2 (tectonic-2 or TECT2) is a type I membrane protein that belongs to the tectonic family, which consists of TCTN1, TCTN2 and TCTN3. Studies in mice suggest that tectonic may be involved in Hedgehog (Hh) signaling, and essential for ciliogenesis. Tectonic is component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. It exists two spilcing isoforms. The first aberrant transcript introduces 104 base pair from intron 13 and would delete 196 original amino acid, introduce two novel amino acids, and prematurely truncate the 697aa protein at residue number 504. The second transcript lacks exon 14 and would delete 195 original amino acids, introduce four novel amino acids, and prematurely truncate the 697 aa protein at residue number 507. The molecular weight of two isoforms are about 50kDa. We detected the 50kDa and 90kDa protein by immunoprecipitaion, but 50kDa protein may be the splicing isoform or non-specific band.