TCP10 Polyclonal antibody

TCP10 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14538-1-AP

Synonyms

---

Validation Data Gallery

• 

  WB analysis of Jurkat using 14538-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14538-1-AP (TCP10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 14538-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14538-1-AP (TCP10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14538-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14538-1-AP (TCP10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14538-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14538-1-AP (TCP10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 14538-1-AP

  Immunofluorescent analysis of HepG2 cells using 14538-1-AP (TCP10 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, HepG2 cells, mouse testis tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14538-1-AP targets TCP10 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TCP10 fusion protein Ag6041
• Full Name: t-complex 10 homolog (mouse)
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC063451
• Gene Symbol: TCP10
• Gene ID (NCBI): 6953
• RRID: AB_2271702
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.