TCN2 Monoclonal antibody, PBS Only

TCN2 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G6D2

Cat no : 66377-1-PBS

Synonyms

TC 2, TC II, TC2, TCII, TCN2, Transcobalamin 2, Transcobalamin II

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Validation Data Gallery

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  WB analysis of HUVEC using 66377-1-Ig (same clone as 66377-1-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 66377-1-Ig (TCN2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66377-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 66377-1-Ig (same clone as 66377-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66377-1-Ig (TCN2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66377-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66377-1-PBS targets TCN2 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: TCN2 fusion protein Ag2825
• Full Name: transcobalamin II; macrocytic anemia
• Calculated Molecular Weight: 400 aa, 44 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC011239
• Gene Symbol: TCN2
• Gene ID (NCBI): 6948
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TCN2 (Transcobalamin-2) is a vitamin B12 (cobalamin; Cbl) binding and transport protein in plasma that facilitates the cellular uptake of the vitamin. TCN2 is secreted in a constitutive fashion in most cells. Plasma TCN2 levels have been reported to be elevated in a number of diverse human diseases. Defects in TCN2 are cause of transcobalamin-2 deficiency. (10714245)