TCN1 Polyclonal antibody

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  WB analysis of human saliva using 16078-1-AP

  human saliva were subjected to SDS PAGE followed by western blot with 16078-1-AP (TCN1 Antibody) at dilution of 1:2000 incubated at 4 degree celsius over night.

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  IHC staining of human testis using 16078-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16078-1-AP (TCN1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 16078-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16078-1-AP (TCN1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human tonsillitis using 16078-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16078-1-AP (TCN1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 16078-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16078-1-AP (TCN1 Antibody) at dilution of 1:50 (under 40x lens).

TCN1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16078-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

TC 1, TC I, TC1, TCI, TCN1, Transcobalamin 1, Transcobalamin I

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Tested Applications

• Positive WB detected in: human saliva tissue
• Positive IHC detected in: human testis tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16078-1-AP targets TCN1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TCN1 fusion protein Ag9072
• Full Name: transcobalamin I (vitamin B12 binding protein, R binder family)
• Calculated Molecular Weight: 433 aa, 48 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC018632
• Gene Symbol: TCN1
• Gene ID (NCBI): 6947
• RRID: AB_2878215
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P20061
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.