TCL1A Polyclonal antibody

TCL1A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 10475-1-AP

Synonyms

TCL1, T-cell leukemia/lymphoma protein 1A, Oncogene TCL-1

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Validation Data Gallery

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  WB analysis of Raji using 10475-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10475-1-AP (TCL1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10475-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10475-1-AP (TCL1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10475-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10475-1-AP (TCL1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 10475-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10475-1-AP (TCL1A antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10475-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10475-1-AP (TCL1A antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10475-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10475-1-AP (TCL1A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 10475-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10475-1-AP (TCL1A Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: Raji cells
• Positive IHC detected in: human tonsillitis tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

10475-1-AP targets TCL1A in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TCL1A fusion protein Ag0786
• Full Name: T-cell leukemia/lymphoma 1A
• Calculated Molecular Weight: 13 kDa
• Observed Molecular Weight: 14-16 kDa
• GenBank Accession Number: BC005831
• Gene Symbol: TCL1A
• Gene ID (NCBI): 8115
• RRID: AB_2255885
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TCL1A, also named as TCL1 and p14 TCL1, enhances cell proliferation, stabilizes mitochondrial membrane potential and promotes cell survival. TCL1A immunodetection is an independent marker of adverse outcome that could be used in routine settings for the management of DLCL patients. TCL1A expression is correlated with shorter time to treatment in chronic lymphocytic leukemia cases and shorter lymphoma-specific survival in mantle cell lymphoma series. It is a potential therapeutic target.

Publications

Species	Application	Title
human	WB	Cell Biol Int; Protection of seven dibenzocyclooctadiene lignans from Schisandra chinensis against serum and glucose deprivation injury in SH-SY5Y cells.; Authors - Qun E