TCIRG1 Recombinant antibody

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  WB analysis using 83351-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83351-6-RR (TCIRG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83351-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83351-6-RR against Human TCIRG1 were performed. The affinity constant is 2.14 nM.

TCIRG1 {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 83351-6-RR

Clone No.240166B2

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

a3, Atp6i, ATP6N1C, ATP6V0A3, OC 116 kDa, OC 116kDa, OC116, OPTB1, Stv1, T cell immune regulator 1, TCIRG1, TIRC7, V ATPase 116 kDa isoform a3, Vph1

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Tested Applications

• Positive WB detected in: SKOV-3 cells, HepG2 cells, Jurkat cells, Daudi cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83351-6-RR targets TCIRG1 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TCIRG1 fusion protein Ag3378
• Full Name: T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3
• Calculated Molecular Weight: 830 aa, 93 kDa
• Observed Molecular Weight: 116 kDa
• GenBank Accession Number: BC018133
• Gene Symbol: TCIRG1
• Gene ID (NCBI): 10312
• RRID: AB_3671009
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q13488
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TCIRG1, also known as V ATPase 116 kDa isoform a3, TIRC7, OPTB1, belongs to the V-ATPase 116 kDa subunit family, TCIRG1 seems to be directly involved in T-cell activation (PubMed:10329006). Alternative splicing results in multiple transcript variants. Isoform long is highly expressed in osteoclastomas. Isoform short is highly expressed in thymus (PMID: 15809087, 24753205). Mutations in TCIRG1 are associated with Osteopetrosis, autosomal recessive 1 (OPTB1)( PMID: 11532986).