TCFL5 Polyclonal antibody

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  WB analysis of mouse testis using 29404-1-AP

  Mouse testis tissue were subjected to SDS PAGE followed by western blot with 29404-1-AP (TCFL5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 29404-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29404-1-AP (TCFL5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 29404-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29404-1-AP (TCFL5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TCFL5 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 29404-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CHA, Cha transcription factor, E2BP 1, E2BP1, Figlb, HPV 16 E2 binding protein 1, TCFL5

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

29404-1-AP targets TCFL5 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TCFL5 fusion protein Ag13479
• Full Name: transcription factor-like 5 (basic helix-loop-helix)
• Calculated Molecular Weight: 483 aa, 52 kDa
• Observed Molecular Weight: 52-60 kDa
• GenBank Accession Number: BC046933
• Gene Symbol: TCFL5
• Gene ID (NCBI): 10732
• RRID: AB_2918297
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UL49
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
	WB	Cell Signal; TCFL5 knockdown sensitizes DLBCL to doxorubicin treatment via regulation of GPX4; Authors - Xueying Lu