TCF1/TCF7 Polyclonal antibody

• Featured Product
• KD/KO Validated

TCF1/TCF7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 14464-1-AP

Synonyms

TCF7, TCF-1, TCF1, TCF 7, TCF 1

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Validation Data Gallery

• 

  WB analysis of mouse thymus using 14464-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14464-1-AP (TCF7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 14464-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14464-1-AP (TCF7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 14464-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14464-1-AP (TCF7 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 14464-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14464-1-AP (TCF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 14464-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14464-1-AP (TCF7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 14464-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14464-1-AP (TCF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse thymus tissue
• Positive IHC detected in: human tonsillitis tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 4 publications below
• IP: See 1 publications below

Product Information

14464-1-AP targets TCF1/TCF7 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TCF1/TCF7 fusion protein Ag5792
• Full Name: transcription factor 7 (T-cell specific, HMG-box)
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC048769
• Gene Symbol: TCF1/TCF7
• Gene ID (NCBI): 6932
• RRID: AB_2878061
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TCF7, also known as TCF1, belongs to the TCF/LEF family. TCF7 is part of the Wnt signaling pathway and plays an important role in the differentiation and self-renewal of memory T cells. It has been found that TCF7 is necessary to revive T cells in response to PD-1 blockade against viral infection or cancer. TCF7 is mainly expressed in T-cells and is also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium. TCF7 has 16 isoforms with the molecular mass of 28-54 kDa (PMID: 34044317).

Publications

Species	Application	Title
mouse	WB	Cell Mol Immunol; TRIM27 maintains gut homeostasis by promoting intestinal stem cell self-renewal; Authors - Jing Wang
human	WB,IHC	Clin Cancer Res; Single-Cell Analyses Reveal Mechanisms of Cancer Stem Cell Maintenance and Epithelial-Mesenchymal Transition in Recurrent Bladder Cancer.; Authors - Huanjun Wang ; KD Validated
human	WB	Clin Transl Med; Long noncoding RNA LINC01606 protects colon cancer cells from ferroptotic cell death and promotes stemness by SCD1-Wnt/β-catenin-TFE3 feedback loop signalling.; Authors - Yajun Luo
mouse	WB	iScience; Suppression of preadipocyte determination by SOX4 limits white adipocyte hyperplasia in obesity; Authors - Ting He
human	WB	J Transl Med; Forkhead Box S1 mediates epithelial-mesenchymal transition through the Wnt/β-catenin signaling pathway to regulate colorectal cancer progression.; Authors - Liang Zhang
human	WB	Front Med (Lausanne); IL-7-Treated Periodontal Ligament Cells Regulate Local Immune Homeostasis by Modulating Treg/Th17 Cell Polarization.; Authors - Xin-Yi Yu