TCEB1 Monoclonal antibody

TCEB1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

2D6D8

Cat no : 68164-1-Ig

Synonyms

EloC, Elongin 15 kDa subunit, Elongin C, SIII, SIII p15, TCEB1

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Validation Data Gallery

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  WB analysis using 68164-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68164-1-Ig (TCEB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of T-47D using 68164-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed T-47D cells using TCEB1 antibody (68164-1-Ig, Clone: 2D6D8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells. HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, rat brain tissue, mouse brain tissue
• Positive IF/ICC detected in: T-47D cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

68164-1-Ig targets TCEB1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TCEB1 fusion protein Ag22601
• Full Name: transcription elongation factor B (SIII), polypeptide 1 (15kDa, elongin C)
• Calculated Molecular Weight: 112 aa, 12 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC013809
• Gene Symbol: TCEB1
• Gene ID (NCBI): 6921
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TCEB1, also named as RNA polymerase II transcription factor SIII subunit C, belongs to the SKP1 family. It mediates the ubiquitination of ECS-E3 ubiquitin-protein ligase complexes. TCEB1 is overexpressed in prostate cancer cell line PC-3 and breast cancer cell line SK-BR-3. TCEB1 also promotes invasion of prostate cancer cells (PMID:18844214).

Publications

Species	Application	Title
human	WB	Cell Death Differ; USP51 facilitates colorectal cancer stemness and chemoresistance by forming a positive feed-forward loop with HIF1A; Authors - Mingchao Mu