TCEA3 Polyclonal antibody

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  WB analysis using 17158-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17158-1-AP (TCEA3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17158-1-AP

  IP result of anti-TCEA3 (IP:17158-1-AP, 4ug; Detection:17158-1-AP 1:500) with HeLa cells lysate 520ug.

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  IF Staining of HeLa using 17158-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17158-1-AP (TCEA3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

TCEA3 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 17158-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Synonyms

Transcription elongation factor S-II protein 3, TFIISH, TFIIS.H, TFIIS

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

17158-1-AP targets TCEA3 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TCEA3 fusion protein Ag10404
• Full Name: transcription elongation factor A (SII), 3
• Calculated Molecular Weight: 348 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC041613
• Gene Symbol: TCEA3
• Gene ID (NCBI): 6920
• RRID: AB_2240352
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75764
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Med Sci Monit; TCEA3 Attenuates Gastric Cancer Growth by Apoptosis Induction.; Authors - Jia Li
human	WB	J Gene Med; Oncogenic tRNA-derived fragment tRF-Leu-CAG promotes tumorigenesis of lung cancer via targeting TCEA3 and increasing autophagy; Authors - Fan Wu
human	WB	J Cell Mol Med; Linoleic Acid Enhances Renal Tubular Epithelial Cells Autophagy Caused by Calcium Oxalate Monohydrate Crystals; Authors - Weiming Ma