TBXAS1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

TBXAS1 Monoclonal Antibody for Indirect ELISA, IHC, WB

Host / Isotype

Mouse / IgG2a

Reactivity

pig, mouse, human

Applications

Indirect ELISA, IHC, WB

Conjugate

Unconjugated

CloneNo.

1F6G6

Cat no : 67371-1-PBS

Synonyms

Hydroperoxy icosatetraenoate dehydratase, EC:5.3.99.5, EC:4.2.1.152, CYP5A1, CYP5

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Validation Data Gallery

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  WB analysis of human peripheral blood platelets using 67371-1-Ig (same clone as 67371-1-PBS)

  Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67371-1-Ig (TBXAS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67371-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 67371-1-Ig (same clone as 67371-1-PBS)

  WB result of TBXAS1 antibody (67371-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TBXAS1 transfected A549 cells. This data was developed using the same antibody clone with 67371-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 67371-1-Ig (same clone as 67371-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67371-1-Ig (TBXAS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67371-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig blood using 67371-1-Ig (same clone as 67371-1-PBS)

  pig blood were subjected to SDS PAGE followed by western blot with 67371-1-Ig (TBXAS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67371-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 67371-1-Ig (same clone as 67371-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 67371-1-Ig (TBXAS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67371-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67371-1-Ig (same clone as 67371-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67371-1-Ig (TBXAS1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67371-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67371-1-Ig (same clone as 67371-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67371-1-Ig (TBXAS1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67371-1-PBS in a different storage buffer formulation.

• 

  FC experiment of A549 using 67371-1-Ig (same clone as 67371-1-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.8 ug TBXAS1 Monoclonal antibody (67371-1-Ig, Clone:1F6G6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67371-1-PBS in a different storage buffer formulation.

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Product Information

67371-1-PBS targets TBXAS1 in Indirect ELISA, IHC, WB applications and shows reactivity with pig, mouse, human samples.

• Tested Reactivity: pig, mouse, human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: TBXAS1 fusion protein Ag29177
• Full Name: thromboxane A synthase 1 (platelet)
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC014117
• Gene Symbol: TBXAS1
• Gene ID (NCBI): 6916
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TBXAS1 catalyzes the conversion of the prostaglandin endoperoxide into thromboxane A2 as a potent vasoconstrictor and inducer of platelet aggregation.It was identified as novel airway fibroblast-specific markers with integrin-8 (ITGA8) (PMID:20435685). It was observed in the epithelium.It is a monomer with the molecular weight of 60 KDa. It also has two variants with 60.5 kDa and 52.3 kDa (PMID:1730669). It also reavels a molecular weight of 55 kDa in humen uterus (PMID:1386258).