TBXAS1 Polyclonal antibody

TBXAS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30215-1-AP

Synonyms

THAS, Hydroperoxy icosatetraenoate dehydratase, EC:5.3.99.5, EC:4.2.1.152, CYP5A1

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Validation Data Gallery

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  WB analysis using 30215-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30215-1-AP (TBXAS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 30215-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30215-1-AP (TBXAS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 30215-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30215-1-AP (TBXAS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: PC-3 cells, THP-1 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30215-1-AP targets TBXAS1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TBXAS1 fusion protein Ag30950
• Full Name: thromboxane A synthase 1 (platelet)
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC014117
• Gene Symbol: TBXAS1
• Gene ID (NCBI): 6916
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBXAS1 catalyzes the conversion of the prostaglandin endoperoxide into thromboxane A2 as a potent vasoconstrictor and inducer of platelet aggregation. It was identified as a novel airway fibroblast-specific marker with integrin-8 (ITGA8) (PMID:20435685). It was observed in the epithelium. It is a monomer with a molecular weight of 60 kDa. It also has two variants with 60.5 kDa and 52.3 kDa (PMID:1730669).