TBX3 Polyclonal antibody

TBX3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

8

Reviews (1)

Cat no : 16741-1-AP

Synonyms

T box 3, T box protein 3, TBX3, TBX3 ISO, UMS, XHL

---

Validation Data Gallery

• 

  WB analysis of HepG2 using 16741-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16741-1-AP (TBX3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16741-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16741-1-AP (TBX3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16741-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16741-1-AP (TBX3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16741-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16741-1-AP (TBX3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16741-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16741-1-AP (TBX3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human placenta using 16741-1-AP

  IP result of anti-TBX3 (IP:16741-1-AP, 4ug; Detection:16741-1-AP 1:500) with human placenta tissue lysate 1520ug.

• 

  IHC staining of human thyroid cancer using 16741-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 16741-1-AP (TBX3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 16741-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 16741-1-AP (TBX3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IP detected in: human placenta tissue
• Positive IHC detected in: human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 5 publications below

Product Information

16741-1-AP targets TBX3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, marmoset
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TBX3 fusion protein Ag9955
• Full Name: T-box 3
• Calculated Molecular Weight: 79 kDa
• Observed Molecular Weight: 79 kDa
• GenBank Accession Number: BC025258
• Gene Symbol: TBX3
• Gene ID (NCBI): 6926
• RRID: AB_2200696
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBX3, also named as T-box protein 3, is a 743 amino acid protein, which contains 1 T-box DNA-binding domain. TBX3 may localize in the nucleus and is widely expressed in various tissues. TBX3 as a transcriptional repressor is involved in developmental processes and probably plays a role in limb pattern formation. TBX3 plays an important role on osteogenic differentiation and proliferation of human mesenchymal stem cell derived from adipose tissue.

Publications

Species	Application	Title
	IHC	Nat Commun; Targeting myeloid derived suppressor cells reverts immune suppression and sensitizes BRAF-mutant papillary thyroid cancer to MAPK inhibitors.; Authors - Peitao Zhang
marmoset	WB	Int J Mol Med; Leukemia inhibitory factor regulates marmoset induced pluripotent stem cell proliferation via a PI3K/‌Akt‑dependent Tbx‑3 activation pathway.; Authors - Minxia Ke
human	WB,IHC	Cell Signal; A new PKCα/β/TBX3/E-cadherin pathway is involved in PLCε-regulated invasion and migration in human bladder cancer cells.; Authors - Du Hong Fei HF
human	IHC	Hum Genomics; Identification of TBX2 and TBX3 variants in patients with conotruncal heart defects by target sequencing.; Authors - Huilin Xie
human	WB,IHC	Exp Cell Res; HOTAIR maintains the stemness of ovarian cancer stem cells via the miR-206/TBX3 axis.; Authors - Yifan Zhang
	WB	Genesis; Homologous recombination efficiency enhanced by inhibition of MEK and GSK3β; Authors - Zhaoyu Lin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Li (Verified Customer) (12-03-2018)

• Applications: Western Blot, Immunohistochemistry,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human cell lines and mouse liver tissue

TBX3 Antibody Western Blot,Immunohistochemistry, validation (1:1000 dilution) in human cell lines and mouse liver tissue (Cat no:16741-1-AP)