TBX2 Polyclonal antibody

TBX2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 22346-1-AP

Synonyms

T box 2, T box protein 2, TBX2

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Validation Data Gallery

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  WB analysis of mouse lung using 22346-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 22346-1-AP (TBX2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 22346-1-AP

  Immunofluorescent analysis of HepG2 cells using 22346-1-AP (TBX2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: mouse lung tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

The immunogen of 22346-1-AP is TBX2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TBX2 fusion protein Ag17860
• Full Name: T-box 2
• Calculated Molecular Weight: 712 aa, 75 kDa
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: BC052566
• Gene Symbol: TBX2
• Gene ID (NCBI): 6909
• RRID: AB_2879083
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse,human	IF	Cell Mol Gastroenterol Hepatol; scRNA-Seq Reveals New Enteric Nervous System Roles for GDNF, NRTN, and TBX3.; Authors - Christina M Wright
human	WB	Commun Biol; Generation and characterization of cardiac valve endothelial-like cells from human pluripotent stem cells.; Authors - LinXi Cheng
human	WB	J Cancer; TBX2 Identified as a Potential Predictor of Bone Metastasis in Lung Adenocarcinoma via Integrated Bioinformatics Analyses and Verification of Functional Assay.; Authors - Huajian Yu