• Featured Product
  • KD/KO Validated

TBRG4 Polyclonal antibody

TBRG4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA, HITS-CLIP

Conjugate

Unconjugated

Cat no : 16245-1-AP

Synonyms

CPR2, FASTKD4, KIAA0948, Protein TBRG4, TBRG4



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, human liver tissue, mouse ovary tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : N/A
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16245-1-AP targets TBRG4 in WB, IHC, IF/ICC, IP, CoIP, ELISA, HITS-CLIP applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBRG4 fusion protein Ag9176
Full Name transforming growth factor beta regulator 4
Calculated Molecular Weight 631 aa, 71 kDa
Observed Molecular Weight 60 kDa, 71 kDa
GenBank Accession NumberBC017235
Gene Symbol TBRG4
Gene ID (NCBI) 9238
RRIDAB_2271487
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBRG4 is also named as CPR2(Cell cycle progression restoration protein 2), FASTKD4(FAST kinase domain-containing protein 4), KIAA0948 and belongs to the FAST kinase family. This protein contains multiple putative leucine zipper domains characteristic of transcription factors and it may play a role in cell cycle progression(PMID:9383053). RT-PCR ELISA detected moderate expression in ovary, and little to no expression in all other tissues and specific brain regions examined(PMID:10231032).TBRG4 has an mature form with MW of 60 kDa produced by transit peptide missing.

Protocols

Product Specific Protocols
WB protocol for TBRG4 antibody 16245-1-APDownload protocol
IHC protocol for TBRG4 antibody 16245-1-APDownload protocol
IF protocol for TBRG4 antibody 16245-1-APDownload protocol
IP protocol for TBRG4 antibody 16245-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,HITS-CLIP

PLoS Genet

The FASTK family proteins fine-tune mitochondrial RNA processing

Authors - Akira Ohkubo
  • KO Validated
humanWB,IHC,IF,CoIP

BMC Cancer

High expression of TBRG4 in relation to unfavorable outcome and cell ferroptosis in hepatocellular carcinoma

Authors - Shanchun Tao
humanWB

Life Sci Alliance

Silica-based solid-phase extraction of cross-linked nucleic acid-bound proteins.

Authors - Claudio Asencio

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Erik (Verified Customer) (02-24-2021)

The antibody used at 1:500 dilution produces a clean and strong band at the expected height for the protein

  • Applications: Western Blot
  • Cell Tissue Type: PANC1 and MIAPACA2 cell lines
TBRG4 Antibody Western Blot validation ( dilution) in PANC1 and MIAPACA2 cell lines (Cat no:16245-1-AP)