CoraLite® Plus 750-conjugated TBP Monoclonal antibody

TBP Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

2H3B2

Cat no : CL750-66166

Synonyms

GTF2D, GTF2D1, SCA17, TATA binding factor, TATA box binding protein, TATA box factor, TATA sequence binding protein, TBP, TF2D, TFIID



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-66166 targets TBP in WB applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TBP fusion protein Ag12383
Full Name TATA box binding protein
Calculated Molecular Weight 338 aa, 38 kDa
Observed Molecular Weight mouse/rat 33-36 kDa and human 37-43kDa
GenBank Accession NumberBC110341
Gene Symbol TBP
Gene ID (NCBI) 6908
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody detects human TBP (~40 kDa) and mouse/rat Tbp (~35 kDa).

Protocols

Product Specific Protocols
WB protocol for CL Plus 750 TBP antibody CL750-66166Download protocol
Standard Protocols
Click here to view our Standard Protocols