TBL2 Monoclonal antibody

TBL2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgA

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D5C2

Cat no : 66307-1-Ig

Synonyms

TBL2, transducin (beta) like 2, Transducin beta like protein 2, WBSCR13, WS betaTRP

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Validation Data Gallery

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  WB analysis of MCF-7 using 66307-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66307-1-Ig (TBL2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66307-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66307-1-Ig (TBL2 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 66307-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66307-1-Ig (TBL2 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 66307-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66307-1-Ig (TBL2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon using 66307-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66307-1-Ig (TBL2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 66307-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66307-1-Ig (TBL2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 66307-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66307-1-Ig (TBL2 Antibody) at dilution of 1:200 (under 10x lens).

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Tested Applications

• Positive WB detected in: MCF-7 cells, COLO 320 cells, Jurkat cells
• Positive IHC detected in: human liver tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66307-1-Ig targets TBL2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgA
• Class: Monoclonal
• Type: Antibody
• Immunogen: TBL2 fusion protein Ag24225
• Full Name: transducin (beta)-like 2
• Calculated Molecular Weight: 446 aa, 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC012938
• Gene Symbol: TBL2
• Gene ID (NCBI): 26608
• RRID: AB_2881689
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Thiophilic affinity chromatograph
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBL2 belongs to a member of the beta-transducin protein family. This gene is commonly deleted in Williams-Beuren syndrome, a developmental disorder with multi-system manifestations caused by haploinsufficiency for contiguous genes deleted in chromosome region 7q11.23. TBL2 encodes a protein with four putative WD40 repeats, which may be involved in some intracellular signaling pathway.