TBK1 Recombinant antibody

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  WB analysis using 83686-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83686-3-RR (TBK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83686-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83686-3-RR (TBK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83686-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83686-3-RR (TBK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

TBK1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 83686-3-RR

Clone No.240769G5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Serine/threonine-protein kinase TBK1, Serine/threonine protein kinase TBK1, NF-kappa-B-activating kinase, NF kappa B activating kinase, EC:2.7.11.1

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Tested Applications

• Positive WB detected in: 4T1 cells, HCT 116 cells, HeLa cells, C6 cells, HSC-T6 cells, RAW 264.7 cells, mouse kidney tissue, HepG2 cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83686-3-RR targets TBK1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TBK1 fusion protein Ag28894
• Full Name: TANK-binding kinase 1
• Observed Molecular Weight: 84 kDa
• GenBank Accession Number: BC034950
• Gene Symbol: TBK1
• Gene ID (NCBI): 29110
• RRID: AB_3671288
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9UHD2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBK1, also named as tumor necrosis factor (TNF) receptor-associated factor NF-kB activator (TANK)-binding kinase 1 (TBK1), NF-kB-activating kinase (NAK), T2K, is a multimeric kinase that modulates inflammation and autophagy. It is a ubiquitously expressed serine-threonine kinase belonging to the 'noncanonical IkB kinases' (IKKs) recognized for its critical role in regulating type I IFN production (PMID: 27211305). And TBK1 is an important player in yet another critical cellular function, autophagy. This antibody is specific for TBK1.