TBCD Recombinant antibody, PBS Only

TBCD Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230209F3

Cat no : 82911-7-PBS

Synonyms

SSD-1, SSD1, PP1096, KIAA0988, Beta-tubulin cofactor D

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Validation Data Gallery

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  WB analysis using 82911-7-RR (same clone as 82911-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82911-7-RR (TBCD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82911-7-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82911-7-RR (same clone as 82911-7-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TBCD antibody (82911-7-RR, Clone: 230209F3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82911-7-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 82911-7-RR (same clone as 82911-7-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug TBCD Recombinant antibody (82911-7-RR, Clone:230209F3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82911-7-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82911-7-RR

  Biolayer interferometry (BLl) kinetic assays of 82911-7-RR against Human TBCD were performed. The affinity constant is 97.6 pM.

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Product Information

82911-7-PBS targets TBCD in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TBCD fusion protein Ag6310
• Full Name: tubulin folding cofactor D
• Calculated Molecular Weight: 133 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC003094
• Gene Symbol: TBCD
• Gene ID (NCBI): 6904
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.