TBCD Polyclonal antibody

TBCD Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 14867-1-AP

Synonyms

Beta tubulin cofactor D, KIAA0988, SSD 1, SSD1, TBCD, tfcD, tubulin folding cofactor D, Tubulin specific chaperone D

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Validation Data Gallery

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  WB analysis using 14867-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14867-1-AP (TBCD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14867-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14867-1-AP (TBCD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14867-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14867-1-AP (TBCD Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse brain using 14867-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14867-1-AP (TBCD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14867-1-AP

  IP result of anti-TBCD (IP:14867-1-AP, 4ug; Detection:14867-1-AP 1:500) with mouse brain tissue lysate 3440ug.

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Tested Applications

• Positive WB detected in: mouse brain tissue, HeLa cells, MCF-7 cells
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 2 publications below

Product Information

14867-1-AP targets TBCD in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TBCD fusion protein Ag6310
• Full Name: tubulin folding cofactor D
• Calculated Molecular Weight: 133 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC003094
• Gene Symbol: TBCD
• Gene ID (NCBI): 6904
• RRID: AB_2199548
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	ACS Chem Neurosci; Proteomic Study on the Mechanism of Arsenic Neurotoxicity in the Rat Cerebral Cortex and the Protective Mechanism of Dictyophora Polysaccharides against Arsenic Neurotoxicity; Authors - Xinglai Zhang
human	IF	Genes Chromosomes Cancer; MYCN overexpression is associated with unbalanced copy number gain, altered nuclear location, and overexpression of chromosome arm 17q genes in neuroblastoma tumors and cell lines.; Authors - Alexandra Kuzyk
mouse	IF	Histochem Cell Biol; Spatio-temporal distribution of tubulin-binding cofactors and posttranslational modifications of tubulin in the cochlea of mice.; Authors - Lukas Juergens
human	WB	Front Genet; Target-Sequencing of Female Infertility Pathogenic Gene Panel and a Novel TUBB8 Loss-of-Function Mutation.; Authors - Hongxia Yuan