TBC1D4 Monoclonal antibody

TBC1D4 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E2H2

Cat no : 68063-1-Ig

Synonyms

Akt substrate of 160 kDa, AS160, DKFZp779C0666, KIAA0603, TBC1 domain family member 4, TBC1 domain family, member 4, TBC1D4

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Validation Data Gallery

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  WB analysis using 68063-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68063-1-Ig (TBC1D4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, HCT 116 cells, LNCaP cells, Jurkat cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68063-1-Ig targets TBC1D4 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TBC1D4 fusion protein Ag20341
• Full Name: TBC1 domain family, member 4
• Calculated Molecular Weight: 1299 aa, 147 kDa
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: BC151239
• Gene Symbol: TBC1D4
• Gene ID (NCBI): 9882
• RRID: AB_2918804
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBC1D4, also named as AS160, KIAA0603, may act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake. The antibody recognizes all the isoform of TBC1D4.