TBC1D24 Polyclonal antibody

TBC1D24 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 25254-1-AP

Synonyms

KIAA1171, TBC1 domain family member 24, TBC1 domain family, member 24, TBC1D24

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Validation Data Gallery

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  WB analysis using 25254-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25254-1-AP (TBC1D24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 25254-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25254-1-AP (TBC1D24 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach using 25254-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25254-1-AP (TBC1D24 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 25254-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25254-1-AP (TBC1D24 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 25254-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25254-1-AP (TBC1D24 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IHC detected in: human stomach tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

25254-1-AP targets TBC1D24 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TBC1D24 fusion protein Ag19380
• Full Name: TBC1 domain family, member 24
• Calculated Molecular Weight: 559 aa, 63 kDa
• Observed Molecular Weight: 60-63 kDa
• GenBank Accession Number: BC127015
• Gene Symbol: TBC1D24
• Gene ID (NCBI): 57465
• RRID: AB_2879990
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Cell Mol Life Sci; The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour.; Authors - Enrico Castroflorio
human,mouse	IHC, WB	PLoS One; Gm14230 controls Tbc1d24 cytoophidia and neuronal cellular juvenescence.; Authors - Takao Morimune

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xin (Verified Customer) (03-15-2022)

A good antibody to detect TBC1D24

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: human postmortem brain lysate

TBC1D24 Antibody Western Blot validation (1:2000 dilution) in human postmortem brain lysate (Cat no:25254-1-AP)