TAU Polyclonal antibody, PBS Only

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  WB analysis of mouse brain using 10274-1-AP (same clone as 10274-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10274-1-AP (TAU antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10274-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 10274-1-AP (same clone as 10274-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10274-1-AP (TAU Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10274-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 10274-1-AP (same clone as 10274-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10274-1-AP (TAU Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10274-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 10274-1-AP (same clone as 10274-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10274-1-AP (TAU antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10274-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 10274-1-AP (same clone as 10274-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10274-1-AP (TAU antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10274-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 10274-1-AP (same clone as 10274-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TAU antibody (10274-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10274-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 10274-1-AP (same clone as 10274-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using TAU antibody (10274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10274-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 10274-1-AP (same clone as 10274-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using TAU antibody (10274-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 10274-1-PBS in a different storage buffer formulation.

TAU Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA

Cat No. 10274-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IF-Fro, ELISA

Synonyms

MAPT, Paired helical filament tau, Microtubule-associated protein tau, MAPTL, DDPAC

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Product Information

10274-1-PBS targets TAU in WB, IHC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TAU fusion protein Ag0354
• Full Name: microtubule-associated protein tau
• Calculated Molecular Weight: 37-46, 79-81 kDa
• Observed Molecular Weight: 45-70 kDa, 100 kDa
• GenBank Accession Number: BC000558
• Gene Symbol: TAU
• Gene ID (NCBI): 4137
• RRID: AB_2139718
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P10636
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Tau (tubulin-associated unit) is a microtubule-associated protein (also known as MAPT), expressed mainly in neurons of the central nervous system. Its primary function is to modulate microtubule dynamics for maintaining axonal cytoskeleton. Various isoforms of Tau exist due to the alternative splicing, and short isoforms around 45-69 kDa and long isoforms around 100-110 kDa have been reported in different literature (8752131,15965697). Present polyclonal anti-Tau antibody was produced by immunizing animals with N-terminal of Tau and can detect approx 45-70 kDa and 100-kDa bands in brain tissues.