TASOR Polyclonal antibody

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  WB analysis using 30188-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30188-1-AP (TASOR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 30188-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30188-1-AP (TASOR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 30188-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30188-1-AP (TASOR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 30188-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30188-1-AP (TASOR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TASOR Polyclonal Antibody for WB, IHC, ELISA

Cat No. 30188-1-AP

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

C3orf63, FAM208A, se89 1, RAP140, KIAA1105

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30188-1-AP targets TASOR in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TASOR fusion protein Ag32757
• Full Name: chromosome 3 open reading frame 63
• Calculated Molecular Weight: 189 kDa
• Observed Molecular Weight: 189 kDa
• GenBank Accession Number: NM_001112736.1
• Gene Symbol: TASOR
• Gene ID (NCBI): 23272
• RRID: AB_2935527
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UK61
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TASOR, also known as FAM208A or C3orf63, is the central subunit of the human silencing hub (HUSH) complex. HUSH regulates deposition of the epigenetic mark H3K9me3. TASOR has a catalytically-inactive PARP domain which is necessary for targeted H3K9me3 deposition (PMID: 33009411). TASOR and the RNA deadenylase CCR4-NOT complex scaffold CNOT1 synergistically repress HIV proviral expression (PMID: 35013187). TASOR affects the genome stability during the cellular division and also interacts with methylation complex in adult tissues (PMID: 34272044).

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Roy (Verified Customer) (06-12-2024)

Could detect TASOR in HeLa cells and Jurkat cells (1/1000 dilution - Overnight incubation at 4°C)

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa, Jurkat