TARS Polyclonal antibody, PBS Only

TARS Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14773-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of A549 using 14773-1-AP (same clone as 14773-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 14773-1-AP (TARS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14773-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 14773-1-AP (same clone as 14773-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 14773-1-AP (TARS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14773-1-PBS in a different storage buffer formulation.

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  IP experiment of A549 using 14773-1-AP (same clone as 14773-1-PBS)

  IP result of anti-TARS (IP:14773-1-AP, 4ug; Detection:14773-1-AP 1:500) with A549 cells lysate 2400ug. This data was developed using the same antibody clone with 14773-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 14773-1-AP (same clone as 14773-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14773-1-AP (TARS Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14773-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 14773-1-AP (same clone as 14773-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14773-1-AP (TARS Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14773-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 14773-1-AP (same clone as 14773-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using TARS antibody 14773-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14773-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 14773-1-AP (same clone as 14773-1-PBS)

  Immunofluorescent analysis of Hela cells, using TARS antibody 14773-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14773-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14773-1-AP (same clone as 14773-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using TARS antibody 14773-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14773-1-PBS in a different storage buffer formulation.

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Product Information

14773-1-PBS targets TARS in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TARS fusion protein Ag6614
• Full Name: threonyl-tRNA synthetase
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 66-72 kDa
• GenBank Accession Number: BC000517
• Gene Symbol: TARS
• Gene ID (NCBI): 6897
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TARS (Threonyl-tRNA synthetase), also known as threonine-tRNA ligase or ThrRS, belongs to the aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetase (aaRS) catalyzes the specific esterification of an amino acid with the 3'-terminal hydroxyl group of its cognate tRNA. Specificity of aminoacylation catalyzed by aaRSs ensures the accurate translation of the genetic code in the first step of protein synthesis (PMID: 26324710). TARS has some isoforms with the molecular mass of 83, 87 kDa and other MW.