TANC2 Polyclonal antibody, PBS Only

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  WB analysis using 31541-1-AP (same clone as 31541-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31541-1-AP (TANC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31541-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 31541-1-AP (same clone as 31541-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TANC2 antibody (31541-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31541-1-PBS in a different storage buffer formulation.

TANC2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 31541-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

ROLSA, rols, KIAA1636, KIAA1148

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Product Information

31541-1-PBS targets TANC2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TANC2 fusion protein Ag35427
• Full Name: tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 2
• Calculated Molecular Weight: 219kDa
• Observed Molecular Weight: 220 kDa
• GenBank Accession Number: NM_001394998
• Gene Symbol: TANC2
• Gene ID (NCBI): 26115
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9HCD6
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Tetratricopeptide repeat, ankyrin repeat, and coiled-coil containing 2 (TANC2), also known as KIAA1148 and KIAA1636, belongs to the TANC family. TANC2 is a large 220 kDa protein consisting of 1990 amino acid residues. Named after its domain architecture, it comprises tetratricopeptide (TPR) and ankyrin repeats (ANK), a coiled-coil domain, and a C-terminal PDZ-interacting motif. TANC2 is widely expressed in the developing human (in excitatory neurons and radial glial cells) and rodent brain as well as in the adult brain as a scaffold in the postsynaptic density (PSD) of excitatory neurons. As such, it facilitates cell signaling downstream of cell surface glutamate receptors through multi-protein complex formation with other PSD proteins (e.g. PSD95, SHANK1), influencing dendritic spine formation and synaptic strength (PMID: 33976205, 34964047).