TAK1 Monoclonal antibody, PBS Only

TAK1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A5B2

Cat no : 67707-1-PBS

Synonyms

MAP3K7, TAK1, TGF beta activated kinase 1, TGF1a

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Validation Data Gallery

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  WB analysis using 67707-1-Ig (same clone as 67707-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67707-1-Ig (TAK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67707-1-Ig (same clone as 67707-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67707-1-Ig (TAK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67707-1-Ig (same clone as 67707-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67707-1-Ig (TAK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67707-1-Ig (same clone as 67707-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67707-1-Ig (TAK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67707-1-Ig (same clone as 67707-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67707-1-Ig (TAK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 67707-1-Ig (same clone as 67707-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67707-1-Ig (TAK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67707-1-Ig (same clone as 67707-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67707-1-Ig (TAK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67707-1-Ig (same clone as 67707-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67707-1-Ig (TAK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 67707-1-Ig (same clone as 67707-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67707-1-Ig (TAK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67707-1-Ig (same clone as 67707-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TAK1 antibody (67707-1-Ig, Clone: 1A5B2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67707-1-Ig (same clone as 67707-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TAK1 antibody (67707-1-Ig, Clone: 1A5B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67707-1-PBS targets TAK1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TAK1 fusion protein Ag21286
• Full Name: mitogen-activated protein kinase kinase kinase 7
• Calculated Molecular Weight: 579 aa, 64 kDa
• Observed Molecular Weight: 67-70 kDa
• GenBank Accession Number: BC017715
• Gene Symbol: MAP3K7
• Gene ID (NCBI): 6885
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named as TAK1 and belongs to the MAP kinase kinase kinase subfamily. It plays an important role in the cascades of cellular responses evoked by changes in the environment. It has been linked to interleukin-1 receptor and tumor necrosis factor receptor signaling (PMID: 16186825). It has 4 isoforms (53-55 kDa, 64-70 kDa and 75-80 kDa) produced by alternative splicing.