TAF3 Polyclonal antibody

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  WB analysis of cells using 18901-1-AP

  WB results of TAF3 (18901-1-AP) with siRNA sample. (140 kDa band absent in siRNA sample).

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  WB analysis using 18901-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18901-1-AP (TAF3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

TAF3 Polyclonal Antibody for WB, ELISA

Cat No. 18901-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

TAFII-140, TAFII140, TAFII 140, TAF140, TAF(II)140

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Tested Applications

• Positive WB detected in: MCF-7 cells, cells, SW480 cells, Y79 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

18901-1-AP targets TAF3 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TAF3 fusion protein Ag13536
• Full Name: TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 140kDa
• Calculated Molecular Weight: 929 aa, 104 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC024661
• Gene Symbol: TAF3
• Gene ID (NCBI): 83860
• RRID: AB_2878567
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5VWG9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TAF3, also named as TAF140, is a 140 kDa subunit of Transcription initiation factor TFIID. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. TAF3 has some isoforms with 727aa (~80 kDa), 622aa and 929aa (105 kDa).

Publications

Species	Application	Title
human	WB	MBio; A targeted RNA interference screen reveals novel epigenetic factors that regulate herpesviral gene expression.; Authors - Hyung Suk Oh