TAF15 Recombinant antibody

• Featured Product
• KD/KO Validated

TAF15 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3L13

Cat no : 82860-1-RR

Synonyms

RNA-binding protein 56, RNA binding protein 56, RBP56, hTAFII68, 68 kDa TATA-binding protein-associated factor

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Validation Data Gallery

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  WB analysis using 82860-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82860-1-RR (TAF15 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 82860-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82860-1-RR (TAF15 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 82860-1-RR

  WB result of TAF15 antibody (82860-1-RR; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAF15 transfected HeLa cells.

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  IHC staining of mouse kidney using 82860-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82860-1-RR (TAF15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 82860-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 82860-1-RR (TAF15 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 82860-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 82860-1-RR (TAF15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 82860-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TAF15 antibody (82860-1-RR, Clone: 3L13 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).CoraLite® Plus 488-Phalloidin (green)

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  Affinity and Kinetic Characterization of 82860-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82860-1-RR against Human TAF15 were performed. The affinity constant is 0.974 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, mouse liver tissue, HEK-293 cells, Jurkat cells, MCF-7 cells, rat brain tissue
• Positive IHC detected in: mouse lung tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82860-1-RR targets TAF15 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TAF15 fusion protein Ag20828
• Full Name: TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa
• Calculated Molecular Weight: 592 aa, 62 kDa
• Observed Molecular Weight: 62-75 kDa
• GenBank Accession Number: BC046099
• Gene Symbol: TAF15
• Gene ID (NCBI): 8148
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TAF15 (TAF2N or TAFII68) is a nuclear protein known to associate with a distinct subpopulation of transcription factor IID (TFIID), a multi-subunit complex that nucleates the pre-initiation complex on promoters of on protein-coding genes. TAF15 harbors a transcriptional activation domain, a RNA recognition motif and many Arg-Gly-Gly repeats that participate in RNA binding. TAF15 along with FUS and EWS constitutes the FET (FUS/EWS/TAF15) protein family of heterogeneous nuclear ribonucleoproteins (hnRNPs) class of RNA binding proteins that are multifunctional proteins implicated in numerous aspects of RNA processing/functions. TAF15 is a nuclear protein that shuttle between cytoplasm-nucleus and as RNA/ssDNA-binding protein, it play specific roles during transcription initiation at distinct promoters and enter the preinitiation complex together with the RNA polymerase II. TAF15 as well as other FET member genes are frequently translocated in sarcomas and rare hematopoietic as well as epithelial cancers. Moreover, dominant mutations in genes coding for four hnRNPs (TDP-43, FUS/TLS, TAF15, and EWS) are found in familial and sporadic cases of ALS and a chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC).