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TAF15 Polyclonal antibody
TAF15 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 25521-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, HEK-293 cells, HeLa cells, mouse brain tissue, SH-SY5Y cells, A549 cells |
Positive IHC detected in | human kidney tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
25521-1-AP targets TAF15 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa |
Calculated Molecular Weight | 62 kDa |
Observed Molecular Weight | 65-70 kDa |
GenBank Accession Number | NM_003487 |
Gene Symbol | TAF15 |
Gene ID (NCBI) | 8148 |
RRID | AB_2880116 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for TAF15 antibody 25521-1-AP | Download protocol |
IHC protocol for TAF15 antibody 25521-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Manohar (Verified Customer) (05-13-2020) | There are multiple bands appearing on western blot. When used with 5% blocking milk and 1:1000 antibody dilution
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