TAF15 Polyclonal antibody

• Featured Product
• KD/KO Validated

TAF15 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, RIP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 24235-1-AP

Synonyms

TAF(II)68, RNA-binding protein 56, RNA binding protein 56, RBP56, hTAFII68

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 24235-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24235-1-AP (TAF15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 24235-1-AP

  WB result of TAF15 antibody (24235-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAF15 transfected HeLa cells.

• 

  IHC staining of human cervical cancer using 24235-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24235-1-AP (TAF15 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 24235-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24235-1-AP (TAF15 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 24235-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 24235-1-AP (TAF15 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 24235-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 24235-1-AP (TAF15 Antibody) at dilution of 1:50.

• 

  IF Staining of HeLa using 24235-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TAF15 antibody (24235-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IHC detected in: human cervical cancer tissue, human kidney tissue, human malignant melanoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• RIP: See 1 publications below

Product Information

24235-1-AP targets TAF15 in WB, IHC, IF/ICC, RIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TAF15 fusion protein Ag20828
• Full Name: TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa
• Calculated Molecular Weight: 592 aa, 62 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC046099
• Gene Symbol: TAF15
• Gene ID (NCBI): 8148
• RRID: AB_2879462
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TAF15 (TAF2N or TAFII68) is a nuclear protein known to associate with a distinct subpopulation of transcription factor IID (TFIID), a multi-subunit complex that nucleates the pre-initiation complex on promoters of on protein-coding genes. TAF15 harbors a transcriptional activation domain, a RNA recognition motif and many Arg-Gly-Gly repeats that participate in RNA binding. TAF15 along with FUS and EWS constitutes the FET (FUS/EWS/TAF15) protein family of heterogeneous nuclear ribonucleoproteins (hnRNPs) class of RNA binding proteins that are multifunctional proteins implicated in numerous aspects of RNA processing/functions. TAF15 is a nuclear protein that shuttle between cytoplasm-nucleus and as RNA/ssDNA-binding protein, it play specific roles during transcription initiation at distinct promoters and enter the preinitiation complex together with the RNA polymerase II. TAF15 as well as other FET member genes are frequently translocated in sarcomas and rare hematopoietic as well as epithelial cancers. Moreover, dominant mutations in genes coding for four hnRNPs (TDP-43, FUS/TLS, TAF15, and EWS) are found in familial and sporadic cases of ALS and a chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC).

Publications

Species	Application	Title
human	WB,RIP	Front Oncol; Long Non-Coding RNA CCAT2 Activates RAB14 and Acts as an Oncogene in Colorectal Cancer.; Authors - Dalu Wang
human,mouse	WB,IHC	Mol Cell Biochem; RNF144A-AS1 stabilizes TAF15 and promotes malignant biological behaviors of skin cutaneous melanoma; Authors - Shude Yang ; KD Validated