TAF12 Polyclonal antibody

• Featured Product
• KD/KO Validated

TAF12 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 12353-1-AP

Synonyms

TAF12, TAF15, TAF2J, TAFII 20/TAFII 15, TAFII20, TAFII20/TAFII15

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Validation Data Gallery

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  WB analysis of Y79 using 12353-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12353-1-AP (TAF12 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Y79 using 12353-1-AP

  IP result of anti-TAF12 (IP:12353-1-AP, 4ug; Detection:12353-1-AP 1:300) with Y79 cells lysate 2000ug.

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Tested Applications

• Positive WB detected in: Y79 cells
• Positive IP detected in: Y79 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• ChIP: See 1 publications below

Product Information

12353-1-AP targets TAF12 in WB, IHC, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TAF12 fusion protein Ag3017
• Full Name: TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC011986
• Gene Symbol: TAF12
• Gene ID (NCBI): 6883
• RRID: AB_2271582
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TAF12, also named TAF15 or TAF2J, is a TBP-associated factor that is contained in Pol I- and Pol II-specific TBP-TAF complexes, it is also a component of the transcription factor IID (TFIID) complex, which is essential for mediating regulation of RNA polymerase transcription. TAF12 plays a key role in regulating eukaryotic gene expression by directly binding promoters and enhancer-bound transactivator proteins. Likewise, TAF12 recruits Gadd45a and the nucleotide excision repair complex to the promoter of rRNA genes leading to active DNA demethylation. Observed MW of TAF12 is from 14-17 kDa and 17-21 kDa.

Publications

Species	Application	Title
mouse	WB	Cancer Cell; A TFIID-SAGA Perturbation that Targets MYB and Suppresses Acute Myeloid Leukemia.; Authors - Yali Xu ; KD Validated
mouse	WB	Cell Metab; Contributions of the measles virus nucleocapsid gene and the SQSTM1/p62(P392L) mutation to Paget's disease.; Authors - Noriyoshi Kurihara
mouse	ChIP,WB	J Bone Miner Res; Role of ATF7-TAF12 interactions in the vitamin D response hypersensitivity of osteoclast precursors in Paget's disease.; Authors - Jumpei Teramachi
mouse, human	WB	J Bone Miner Res; TBK1 mediates critical effects of measles virus nucleocapsid protein (MVNP) on pagetic osteoclast formation.; Authors - Sun Quanhong Q
mouse	WB	J Bone Miner Res; Increased IL-6 expression in osteoclasts is necessary but not sufficient for the development of Paget's disease of bone.; Authors - Jumpei Teramachi
human	WB	Mol Cell Biol; The general transcription factor TAF7 is essential for embryonic development but not essential for the survival or differentiation of mature T cells.; Authors - Gegonne Anne A

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