HepG2 cells were subjected to SDS PAGE followed by western blot with 16114-1-AP (TAF11 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
HepG2 cells were subjected to SDS PAGE followed by western blot with 16114-1-AP (TAF11 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16114-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16114-1-AP (TAF11 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 16114-1-AP (TAF11 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16114-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16114-1-AP (TAF11 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 16114-1-AP (TAF11 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 16114-1-AP
IP result of anti-TAF11 (IP:16114-1-AP, 4ug; Detection:16114-1-AP 1:300) with HeLa cells lysate 1600 ug.
IP result of anti-TAF11 (IP:16114-1-AP, 4ug; Detection:16114-1-AP 1:300) with HeLa cells lysate 1600 ug.
IHC staining of human colon cancer using 16114-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16114-1-AP (TAF11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16114-1-AP (TAF11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 16114-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16114-1-AP (TAF11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16114-1-AP (TAF11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 16114-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16114-1-AP (TAF11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16114-1-AP (TAF11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 16114-1-AP
Immunofluorescent analysis of HepG2 cells using 16114-1-AP (TAF11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of HepG2 cells using 16114-1-AP (TAF11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:200-1:1000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
16114-1-AP targets TAF11 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
TAF11, also named as Transcription initiation factor TFIID 28 kDa subunit, is a 211 amino acid protein, which Belongs to the TAF11 family. TAF11 as a core TAFII present in both of the previously described TFIID species which either lack or contain TAFII30 . The calcualted molecular weight of TAF11 is 23 kDa, but modified TAF11 is about 28kDa. ( PMID: 10336414)
HepG2 cells were subjected to SDS PAGE followed by western blot with 16114-1-AP (TAF11 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16114-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16114-1-AP (TAF11 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16114-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16114-1-AP (TAF11 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human colon cancer using 16114-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16114-1-AP (TAF11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 16114-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16114-1-AP (TAF11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 16114-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16114-1-AP (TAF11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HeLa using 16114-1-AP
IP result of anti-TAF11 (IP:16114-1-AP, 4ug; Detection:16114-1-AP 1:300) with HeLa cells lysate 1600 ug.
IF/ICC Figures
IF Staining of HepG2 using 16114-1-AP
Immunofluorescent analysis of HepG2 cells using 16114-1-AP (TAF11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
TAF11 Polyclonal antibody
Catalog Number
16114-1-AP
Citations
-
Dilutions
WB : 1:200-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:20-1:200
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
