TAF10 Polyclonal antibody

TAF10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20346-1-AP

Synonyms

STAF28, TAF(II)30, TAF10, TAF2A, TAF2H, TAFII 30, TAFII30

---

Validation Data Gallery

• 

  WB analysis of mouse testis using 20346-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20346-1-AP (TAF10 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 20346-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20346-1-AP (TAF10 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 20346-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20346-1-AP (TAF10 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:500
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 20346-1-AP is TAF10 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TAF10 fusion protein Ag14153
• Full Name: TAF10 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 30kDa
• Calculated Molecular Weight: 218 aa, 22 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC012088
• Gene Symbol: TAF10
• Gene ID (NCBI): 6881
• RRID: AB_2878678
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Biochem Pharmacol; Calcium saccharate/DUSP6 suppresses renal cell carcinoma glycolytic metabolism and boosts sunitinib efficacy via the ERK-AKT pathway; Authors - Huan Liu