TAF1-Specific Polyclonal antibody

TAF1-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20260-1-AP

Synonyms

BA2R, CCG1, CCGS, Cell cycle gene 1 protein, DYT3, KAT4, N TAF1, NSCL2, OF, P250, TAF(II)250, TAF1, TAF1-Specific, TAF2A, TAFII 250, TAFII250, TBP associated factor 250 kDa

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Validation Data Gallery

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  WB analysis using 20260-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20260-1-AP (TAF1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20260-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20260-1-AP (TAF1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 20260-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:600 (under 40x lens).

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  IHC staining of human prostate cancer using 20260-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human prostate cancer using 20260-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 20260-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 20260-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human prostate cancer using 20260-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:500 (under 10x lens).

• 

  IHC staining of human prostate cancer using 20260-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:500 (under 40x lens).

• 

  IHC staining of human prostate cancer using 20260-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:600 (under 10x lens).

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Tested Applications

• Positive WB detected in: HEK-293T cells, mouse brain tissue, HeLa cells, Jurkat cells, K-562 cells
• Positive IHC detected in: human prostate cancer tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

20260-1-AP targets TAF1-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa
• Calculated Molecular Weight: 213 kDa
• Observed Molecular Weight: 250-260 kDa, 50 kDa
• GenBank Accession Number: NM_138923
• Gene Symbol: TAF1
• Gene ID (NCBI): 6872
• RRID: AB_10693532
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TAF1, also named as BA2R, CCG1, CCGS, TAF2A, p250, Cell cycle gene 1 protein, TAFII250 and DYT3, belongs to the TAF1 family. TAF1 is the largest component and core scaffold of the TFIID basal transcription factor complex. It contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. TAF1 phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. TAF1 phosphorylates GTF2A1 and GTF2F1 on Ser residues. It possesses DNA-binding activity. It is essential for progression of the G1 phase of the cell cycle. Defects in TAF1 are the cause of dystonia type 3 (DYT3) which also called X-linked dystonia-parkinsonism (XDP). The antibody is specific to TAF1. It has several isoforms with predicted MW's of 200-250 kDa and 170-180 kDa, it also could get about 50 kDa band which is caused by the lysate preparation.

Publications

Species	Application	Title
human	WB	J Extracell Vesicles; Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours.; Authors - Xiaoyu Yu
human	WB	J Clin Invest; Long noncoding RNA HITT coordinates with RGS2 to inhibit PD-L1 translation in T cell immunity; Authors - Qingyu Lin