TADA2L Recombinant antibody

TADA2L Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241508D8

Cat no : 84227-2-RR

Synonyms

hADA2, ADA2-like protein, ADA2A, ADA2 like protein, ADA2

---

Validation Data Gallery

• 

  WB analysis using 84227-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84227-2-RR (TADA2L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 84227-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84227-2-RR against Human TADA2L were performed. The affinity constant is below 1 pM.

---

Tested Applications

• Positive WB detected in: HeLa cells, K562 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84227-2-RR is TADA2L Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TADA2L fusion protein Ag1817
• Full Name: transcriptional adaptor 2 (ADA2 homolog, yeast)-like
• Calculated Molecular Weight: 51 aa, 5 kDa
• Observed Molecular Weight: 35-50 kDa
• GenBank Accession Number: BC011753
• Gene Symbol: TADA2L
• Gene ID (NCBI): 6871
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TADA2A is a subunit of the ADA2A-containing (ATAC) histone acetyltransferase complex, which with histone acetyltransferase activity on histones H3 and H4. It is required for the function of some acidic activation domains, which activate transcription from a distant site. It has a role in chromatin remodeling by binding at the linker region between neighbouring unclesomes of dinucleosomes [PMID:19103755].