TACSTD2/TROP2 Polyclonal antibody, PBS Only

TACSTD2/TROP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27360-1-PBS

Synonyms

TACSTD2, M1S1, GA733-1, GA733 1, GA733

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Validation Data Gallery

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  WB analysis of HaCaT using 27360-1-AP (same clone as 27360-1-PBS)

  HaCaT cells were subjected to SDS PAGE followed by western blot with 27360-1-AP (TACSTD2 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27360-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 27360-1-AP (same clone as 27360-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27360-1-AP (TACSTD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27360-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 27360-1-AP (same clone as 27360-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27360-1-AP (TACSTD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27360-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 27360-1-AP (same clone as 27360-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27360-1-AP (TACSTD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27360-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 27360-1-AP (same clone as 27360-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27360-1-AP (TACSTD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27360-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HaCaT using 27360-1-AP (same clone as 27360-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using TACSTD2/TROP2 antibody (27360-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27360-1-PBS in a different storage buffer formulation.

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Product Information

27360-1-PBS targets TACSTD2/TROP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TACSTD2/TROP2 fusion protein Ag25769
• Full Name: tumor-associated calcium signal transducer 2
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 45-55 kDa
• GenBank Accession Number: BC009409
• Gene Symbol: TROP2
• Gene ID (NCBI): 4070
• RRID: AB_2918122
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Trophoblast cell surface antigen 2 (TROP2), also named as TACSTD2, is a transmembrane glycoprotein. TROP2 plays a role in transducing intracellular calcium signals. It is expressed in trophoblast cells, cornea and multi-stratified epithelia. TROP2 is overexpressed in most carcinomas and is involved in cancer proliferation, migration, invasion, and metastasis. Congenital mutations of TROP2 cause a rare autosomal recessive disease which may lead to blindness-the gelatinous drop-like corneal disease (PMID: 35688908).