TACC1 Polyclonal antibody

TACC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13862-1-AP

Synonyms

Transforming acidic coiled-coil-containing protein 1, Taxin-1, Taxin1, KIAA1103, Ga55

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Validation Data Gallery

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  WB analysis of MCF-7 using 13862-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13862-1-AP (TACC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13862-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13862-1-AP (TACC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 13862-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13862-1-AP (TACC1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 13862-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13862-1-AP (TACC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 13862-1-AP

  Immunofluorescent analysis of HeLa cells using 13862-1-AP (TACC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 13862-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TACC1 antibody (13862-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: MCF7 cells, HeLa cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

13862-1-AP targets TACC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TACC1 fusion protein Ag4842
• Full Name: transforming, acidic coiled-coil containing protein 1
• Calculated Molecular Weight: 805 aa, 88 kDa
• Observed Molecular Weight: 110-130 kDa
• GenBank Accession Number: BC041391
• Gene Symbol: TACC1
• Gene ID (NCBI): 6867
• RRID: AB_2255719
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Cell Death Discov; Loss of TACC1 variant25 inducing cell proliferation and suppressing autophagy in head and neck squamous carcinoma.; Authors - Pan Xu