TAC1 Polyclonal antibody

TAC1 Polyclonal Antibody for IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 28599-1-AP

Synonyms

TACI, TAC 1, Protachykinin-1, Protachykinin 1, C-terminal-flanking peptide

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Validation Data Gallery

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  IHC staining of mouse brain using 28599-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28599-1-AP (TAC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 28599-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28599-1-AP (TAC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hypothalamus using 28599-1-AP

  Immunohistochemical analysis of paraffin-embedded human hypothalamus tissue slide using 28599-1-AP (TAC1 antibody) at dilution of 1:200 (under 10x lens)..

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  IHC staining of human hypothalamus using 28599-1-AP

  Immunohistochemical analysis of paraffin-embedded human hypothalamus tissue slide using 28599-1-AP (TAC1 antibody) at dilution of 1:200 (under 40x lens)..

• 

  IF Staining of mouse brain using 28599-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TAC1 antibody (28599-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 28599-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TAC1 antibody (28599-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of PC-12 using 28599-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using TAC1 antibody (28599-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: mouse brain tissue, human hypothalamus tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue
• Positive IF/ICC detected in: PC-12 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28599-1-AP targets TAC1 in IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TAC1 fusion protein Ag23477
• Full Name: tachykinin, precursor 1
• Calculated Molecular Weight: 129 aa, 15 kDa
• GenBank Accession Number: BC018047
• Gene Symbol: TAC1
• Gene ID (NCBI): 6863
• RRID: AB_2881178
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TAC1, also known as Protachykinin-1, is a gene that encodes for a family of neuropeptides known as tachykinins. These peptides are characterized by their ability to rapidly stimulate contraction of intestinal muscle, hence the name "tachykinins". The TAC1 gene and its products are widely distributed in the nervous system, with α-TAC1 mRNA localized in numerous neurons of the brain. β-TAC1 and γ-TAC1 are predominantly expressed in intrinsic enteric neurons and sensory neurons. Additionally, the expression of TAC1, SP, and NKA is observed in various peripheral neurons and non-neural tissues such as the salivary gland, heart, skin, spleen, adrenal gland, and more.