Syntenin-1 Polyclonal antibody, PBS Only

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  WB analysis using 22399-1-AP (same clone as 22399-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22399-1-AP (Syntenin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22399-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 22399-1-AP (same clone as 22399-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 22399-1-AP (Syntenin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22399-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 22399-1-AP (same clone as 22399-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22399-1-AP (Syntenin-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22399-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 22399-1-AP (same clone as 22399-1-PBS)

  IP result of anti-Syntenin-1 (IP:22399-1-AP, 3ug; Detection:22399-1-AP 1:1000) with HeLa cells lysate 2500ug. This data was developed using the same antibody clone with 22399-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 22399-1-AP (same clone as 22399-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22399-1-AP (Syntenin-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22399-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 22399-1-AP (same clone as 22399-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22399-1-AP (Syntenin-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22399-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 22399-1-AP (same clone as 22399-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart slide using 22399-1-AP (Syntenin-1 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22399-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 22399-1-AP (same clone as 22399-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart slide using 22399-1-AP (Syntenin-1 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22399-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 22399-1-AP (same clone as 22399-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22399-1-AP (Syntenin-1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22399-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 22399-1-AP (same clone as 22399-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22399-1-AP (Syntenin-1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22399-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 22399-1-AP (same clone as 22399-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Syntenin-1 antibody (22399-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 22399-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Syntenin-1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 22399-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only

PBS and Azide

PBS Only

Conjugate:  Unconjugated

Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Product Information

22399-1-PBS targets Syntenin-1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Syntenin-1 fusion protein Ag18078
• Full Name: syndecan binding protein (syntenin)
• Calculated Molecular Weight: 298 aa, 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC113674
• Gene Symbol: Syntenin-1
• Gene ID (NCBI): 6386
• RRID: AB_2879100
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O00560
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Syntenin-1, also known as SDCBP (syndecan binding protein) and MDA-9 (melanoma differentiation-associated protein 9), was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. Syntenin-1 is a PDZ-domain-containing molecule that has many interaction partners, and regulates transmembrane-receptor trafficking, cell adhesion, tumor-cell metastasis and neuronal-synapse function. Syntenin-1 is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus.