Syntaxin 16 Monoclonal antibody, PBS Only

Syntaxin 16 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4A9D8

Cat no : 66775-1-PBS

Synonyms

hsyn16, STX16, SYN16, syntaxin 16

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Validation Data Gallery

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  WB analysis using 66775-1-Ig (same clone as 66775-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66775-1-Ig (Syntaxin 16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66775-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 66775-1-Ig (same clone as 66775-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66775-1-Ig (Syntaxin 16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66775-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 66775-1-Ig (same clone as 66775-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66775-1-Ig (Syntaxin 16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66775-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66775-1-Ig (same clone as 66775-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66775-1-Ig (Syntaxin 16 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66775-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66775-1-Ig (same clone as 66775-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66775-1-Ig (Syntaxin 16 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66775-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66775-1-Ig (same clone as 66775-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66775-1-Ig (Syntaxin 16 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66775-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66775-1-Ig (same clone as 66775-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66775-1-Ig (Syntaxin 16 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66775-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66775-1-Ig (same clone as 66775-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66775-1-Ig (Syntaxin 16 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66775-1-PBS in a different storage buffer formulation.

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  IF Staining of Neuro-2a using 66775-1-Ig (same clone as 66775-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using Syntaxin 16 antibody (66775-1-Ig, Clone: 4A9D8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66775-1-PBS in a different storage buffer formulation.

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Product Information

66775-1-PBS targets Syntaxin 16 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Syntaxin 16 fusion protein Ag27926
• Full Name: syntaxin 16
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 37-40 kDa
• GenBank Accession Number: BC019042
• Gene Symbol: Syntaxin 16
• Gene ID (NCBI): 8675
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Syntaxin 16 (STX16), also named as SYN16, belongs to the syntaxin family. Syntaxins are target membrane SNAREs(Soluble NSF attachment protein receptors) which are membrane components that determine the specificity of the docking and fusion of vesicles to target membranes. STX16 is localized to the Golgi apparatus.