Syntaxin 12 Polyclonal antibody, PBS Only

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  WB analysis using 14259-1-AP (same clone as 14259-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 14259-1-AP (same clone as 14259-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

• 

  WB analysis of BxPC-3 using 14259-1-AP (same clone as 14259-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 14259-1-AP (same clone as 14259-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 14259-1-AP (same clone as 14259-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 14259-1-AP (same clone as 14259-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 14259-1-AP (same clone as 14259-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 14259-1-AP (same clone as 14259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 14259-1-AP (same clone as 14259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 14259-1-AP (same clone as 14259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 14259-1-AP (same clone as 14259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 14259-1-AP (same clone as 14259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 14259-1-AP (same clone as 14259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 14259-1-AP (same clone as 14259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 14259-1-AP (same clone as 14259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14259-1-AP (same clone as 14259-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using STX12 antibody 14259-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 14259-1-AP (same clone as 14259-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using STX12 antibody 14259-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Syntaxin 12 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 14259-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Syntaxin-12, STX12

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Product Information

14259-1-PBS targets Syntaxin 12 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Syntaxin 12 fusion protein Ag5532
• Full Name: syntaxin 12
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 33-39 kDa, 66 kDa
• GenBank Accession Number: BC046999
• Gene Symbol: Syntaxin 12
• Gene ID (NCBI): 23673
• RRID: AB_2198222
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86Y82
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Syntaxin 12 (STX12), also known as STX13 or STX14, is a 276 amino acid single-pass type IV membrane protein that belongs to the syntaxin family. It is a broadly and differentially expressed SNARE protein, highly enriched in brain tissue. STX12 is localized to early and recycling endosomes and is involved in the recycling of plasma membrane proteins. It also has a role in the trafficking of MMP during the degradation of ECM substrates and subsequent cellular invasion. (PMID: 9507000; 9553086; 19910495) This antibody recognizes a major 39 kDa band of endogenous STX12. In some cases, two additional bands of 33 kDa and 66 kDa could also be detected, which most likely represent a degradation product and a SNARE complex respectively (PMID: 9817754).