Syntaxin 10 Monoclonal antibody, PBS Only (Detector)

Syntaxin 10 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B1C3

Cat no : 66821-1-PBS

Synonyms

Syntaxin-10, Syn10, STX10, 1B1C3

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Validation Data Gallery

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  WB analysis using 66821-1-Ig (same clone as 66821-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66821-1-Ig (Syntaxin 10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66821-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66821-1-Ig (same clone as 66821-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Syntaxin 10 antibody (66821-1-Ig, Clone: 1B1C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66821-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51170-3

  Cytometric bead array standard curve of MP51170-3, STX10 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66821-5-PBS. Detection antibody: 66821-1-PBS. Standard:Ag27861. Range: 1.563-100 ng/mL.

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Product Information

66821-1-PBS targets Syntaxin 10 as part of a matched antibody pair:

MP51170-3: 66821-5-PBS capture and 66821-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Syntaxin 10 fusion protein Ag27861
• Full Name: syntaxin 10
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC017237
• Gene Symbol: Syntaxin 10
• Gene ID (NCBI): 8677
• RRID: AB_2882164
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Syntaxin 10 (STX10), also named as SYN10, belongs to the syntaxin family. Syntaxins are target membrane SNAREs(Soluble NSF attachment protein receptors) which are membrane components that determine the specificity of the docking and fusion of vesicles to target membranes.